Cytochalasin b glucose uptake

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WebMay 23, 2003 · Glucose levels dropped even in the presence of the transport inhibitor cytochalasin B, indicating rapid metabolism. Consistently, the addition of 2 … WebCytochalasin B is a cell-permeable fungal toxin which binds to the barbed end of actin filaments inhibiting both the association and dissociation of subunits. It causes the disruption of actin filaments and inhibition of actin polymerization. ... Effect of cytochalasin B on glucose uptake, utilization, oxidation and insulinotropic action in ...

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WebThe widely expressed mammalian glucose transporter, GLUT1, can be acutely activated in L929 fibroblast cells by a variety of conditions, including glucose deprivation, or treatment with various respiration inhibitors.Known thiol reactive compounds including phenylarsine oxide and nitroxyl are the fastest acting stimulators of glucose uptake, implicating … WebMay 13, 2024 · Impairment of cellular glucose uptake due to compromised insulin ... we report the near atomic resolution cryo-EM structures of GLUT4 bound to a small … north carolina medicaid reimbursement rates

Glucose uptake in brown fat cells is dependent on mTOR …

WebIn vitro and in vivo study of phloretin-induced apoptosis in human liver cancer cells involving inhibition of type II glucose transporter. Chih Hsiung Wu, Yuan Soon Ho, Chia Yi Tsai, Ying Jan Wang, How Tseng, Po Li Wei, Chia Hwa Lee, Ren Shyan Liu, Shyr Yi Lin. WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. This coincided with a marked decrease in D - [U- 14 C] glucose oxidation and suppression of the stimulant action of D -glucose upon insulin release. WebCytochalasin B, which is often used as an inhibitor of actin-based motility systems, is also a very potent competitive inhibitor of Đ-glucose uptake into mammalian cells. When red blood cell ghosts (yes it's a real term, these are RBCs emptied of their cytoplasm) are incubated when 'H-cytochalasin and then irradiated with ultraviolet light, the how to reset an iphone xr with itunes

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In vivo imaging of the dynamics of glucose uptake in the …

WebAbstract. Cytochalasin B (17-3 microM) virtually abolished 3-O-methyl-D- [U-14C]glucose uptake and D- [5-3H]glucose utilization in tumoral insulin-producing cells of the RINm5F … WebCytochalasin B, CAS: 14930-96-2, is a cell-permeable fungal toxin used in actin polymerization studies and cytological research.. Cited in 16 publications ... Effect of cytochalasin B on glucose uptake, utilization, oxidation and insulinotropic action in tumoral insulin-producing cells. Malaisse, WJ., et al. 1987. Cell Biochem Funct. 5: 183-7. north carolina medicaid incontinence suppliesWebJul 1, 1974 · Cytochalasin B in this concentration suppresses multiplication rates in a nutrient medium consisting of 2 % proteose peptone, but multiplication is unaffected if … how to reset an iphone xr that is frozen

"WebApr 14, 2016 · Glucose uptake for GlcP Se was measured one minute after initiation of transport with 30 μM C 14 -glucose in the presence of 2 mM … " - Cytochalasin b glucose uptake

Cytochalasin b glucose uptake

Glucose Transport and Glucose Transporters in Muscle and Their ...

WebCytochalasin B (50µM) was added to determine assay background. Glucose Uptake-Glo™ Assay We have developed a luminescent glucose uptake assay that is based on the same principle as the aforementioned … WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. …

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WebMar 5, 2010 · When expressed in S. cerevisae, rat GLUT1 showed a Km for D -glucose of 3.4 mM and transport was inhibited by cytochalasin B (IC50 = 0.44 μM), HgCl 2 (IC50 = 3.5 μM), phloretin (IC50 = 49 μM), and … WebApr 7, 2024 · It might be related to the F-actin disruption caused by ES, since the distribution of GLUT1 on cell surface could be changed from clusters to dispersed states when the actin cytoskeleton depolymerized by cytochalasin B. We further examined the glucose uptake capacity of cells after the ES using fluorescence glucalogue (2-NBDG), which has the ...

WebNov 11, 2008 · Cytochalasin B (10 μmol/L) and phloretin (20 μmol/L) inhibited glucose uptake 85 ± 1% and 84 ± 18%, respectively. In contrast, the selective GLUT4 inhibitor … WebMay 1, 1972 · Abstract. A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-d-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, …

WebIn erythrocytes, adipocytes, and chick embryo fibroblasts, the Ki for glucose uptake inhibition and the Kd for cytochalasin B binding range from 0.1 to 0.4 µM. Values for hepatocytes and liver membranes are an order of magnitude higher … WebThis dependence results in up-regulation of glucose transporter expression, especially hGLUT1. The bound inhibitors include the natural compound cytochalasin B and two of a series of previously undescribed organic compounds that bind in the submicromolar range.

WebCytochalasin B (1 mg; Sigma-Aldrich; C6762). Add 2 ml DMSO into a vial with 1 mg cytochalasin B (500 μg; 100× CB stock solution). Divide into small tubes (10–20 μl) and store at − 80 °C. • TSA (1 mg; Sigma-Aldrich; T8552).

WebJun 18, 2024 · The glucose transporter GLUT1, a plasma membrane protein that mediates glucose homeostasis in mammalian cells, is responsible for constitutive uptake of glucose into many tissues and organs. Many studies have focused on its vital physiological functions and close relationship with diseases. how to reset an lg stylo 6WebNov 11, 2008 · Non-carrier-mediated uptake was determined in parallel wells containing 10 μmol/L cytochalasin B. Protein concentration was measured by the modified Bradford protein assay. Cell-associated radioactivity was determined by scintillation counting. The mean counts/min were expressed as pmol/min/mg protein from determinations done in … how to reset anker power bankWeb2.2 Assaying Insulin-Stimulated Glucose Uptake. 1. Serum-free media: DMEM (with 4.5 g/L glucose, l-glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin … north carolina medicaid statuteWeb(B) Glucose uptake was assayed by incubation of control, cytochalasin B-, and cytochalasin D-treated Jurkat cells in the presence of 2-deoxy-D [1-3H]glucose (5 μM) … how to reset anker powerconfWebFeb 27, 2024 · The present study was conducted to explore the mechanism of nano-Zn absorption and its influence on lipid metabolism in the intestine of yellow catfish Pelteobagrus fulvidraco. Compared to ZnSO4, dietary nano-Zn addition increased the triglyceride (TG) content, enzymatic activities of malic enzyme (ME) and fatty acid … north carolina medicaid providers listWebApr 23, 2015 · Moreover, cytochalasin B, a nonspecific glucose transport inhibitor, also strongly inhibited the uptake of 14 C-glucose, 14 C-2DG and 2DG-IR in neuronal and astrocytic cultures . north carolina medicaid spend down rulesWebMay 1, 1972 · A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-D-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is achieved at very low... north carolina medicaid sign in