E2a antibody
WebJan 25, 2015 · Linking the heavy chain (HC) and light chain (LC) genes required for monoclonal antibodies (mAb) production on a single cassette using 2A peptides allows control of LC and HC ratio and reduces non-expressing cells. Four 2A peptides derived from the foot-and-mouth disease virus (F2A), equine rhinitis A virus (E2A), porcine … WebThe guide RNA in the E2A Lentiviral Activation Particles (h2) encodes a target-specific 20 nt guide RNA that differs from the guide RNA in E2A Lentiviral Activation Particles (h) Following transfection, gene activation efficiency can be assayed by WB, IF or IHC using antibody: E2A Antibody (G-2): sc-133075
E2a antibody
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WebThe translocation results in the transcription of a unique chimeric protein E2A-Pbx1 with an amino acid sequences not present on E2A or Pbx alone. E2A-Pbx1 is found only in cells with the t (1,19) translocation. It is detectable in the nucleus of 697 cell line (a pre-B cell line). E2A-Pbx1 is considered to be an oncoprotein, however, the ... WebPolyclonal Antibody for studying E2A. Cited in 7 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, E2A Antibody (CST #4865) is ready to ship.
WebAll lanes : Anti-TCF3 / E2A antibody (ab69999) at 1/500 dilution Lane 1 : Extracts from 293 cells, minus immunising peptide Lane 2 : Extracts from 293 cells, plus immunising … WebTCF3 (E2A) is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell …
WebThis 2A Peptide Antibody (3H4) was developed against KLH-coupled synthetic peptide corresponding to 2A peptide of Thosea asigna virus (CGDVEENPG) Specificity. This 2A Peptide Antibody (3H4) peptide … WebFeb 2, 1999 · The anti-human E2A antibody G98–271, the antibody 382.6 (specific for E12, HEB, and E2-2), and goat anti-mouse IgG-fluorescein isothiocyanate (FITC) were obtained from PharMingen. Hexadimethrine bromide (Polybrene) was purchased from Sigma and DOTAP was purchased from Boehringer-Mannheim.
WebApr 8, 2024 · I ChIP assays were performed in A549 cells using a specific E2A antibody (anti-E47) or a control rabbit normal IgG (anti-IgG) and primers specific for P1, P2, and P3. A randomly segment (−40 to ...
WebE2A Antibody. 4865 is a primary antibody from Cell Signaling Technology. 7 Citations Host: Rabbit Clonality: Polyclonal Validations: None Available Applications: WB. Reactivity: Homo sapiens (Human) Mouse anti Human Vitamin D Receptor:Preservative Free. MCA3543Z is a primary antibody from Bio-Rad (Formerly AbD Serotec) irons nephew in house of gucci crosswordWebE2A is a member of the E-protein family of transcription factors, a subclass of basic helix-loop-helix (bHLH) proteins that bind specifically to E-box … irons near meWebWestern Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000. Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585] - Jurkat whole cell extract A. 35 ug Jurkat whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of TCF3 protein by 4 ug TCF3 ... port whitbyWebAll lanes : Anti-TCF3 / E2A antibody [7H3] (ab243152) at 0.537 µg/ml Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate … port whitby marine storeWebAntibodies that detect TCF3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Flow Cytometry. These antibodies target TCF3 in Human, Mouse, Rat and Non-human primate samples. Our TCF3 polyclonal, monoclonal and recombinant monoclonal antibodies are … irons natural stateport whiskyWebTCF-3/E2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44061) at 10 µg/mL for 3 hours at room temperature. Cells were … irons mystic menu