Fitc-conjugated pac-1

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August undefined, 2024

WebThe PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets. The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, α IIb β 3) complex of activated platelets at or near the platelet fibrinogen receptor. WebMutated KRAS proteins are often constitutively active, resulting in the dysregulated growth and transformation pathways that promote pancreatic cancer growth, survival, and metastasis. Proteintech’s KRAS antibody is a favorite among researchers worldwide, cited in nearly 100 publications. Immunohistochemical analysis of paraffin-embedded ...

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WebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. WebSep 18, 2024 · FITC-conjugated anti-human CD62P (P-selectin) and FITC-conjugated anti-human PAC-1 were purchased from BioLegend (California, USA). Fluo-3 AM calcium indicators and lactate dehydrogenase (LDH) … rbl bank owned by

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WebThe antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. ... a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue … WebUse RGDS with conjugated PAC-1 antibody NOTE: Some commercial preparations can include lipopolysaccharide (LPS), a potent platelet activator. FACS brand flow cytometer. Refer to the appropriate instrument user’s guide for detailed information. ... stain with PAC-1 FITC/CD62P PE/CD61 PerCP in the presence of RGDS to inhibit PAC-1 binding ... WebFITC Protocols Fluorescein Isothiocyanate (FITC) - conjugated Antibodies GFP Protocols › Immunoprecipitation Protocols › Membrane Protein Protocols › Protein Expression Protocols › Protein Isolation Protocols › Protein Purification Protocols › SDS PAGE Protocols › rbl bank od against fd

PAC-1 antibody (FITC) anti PAC-1 antibody (FITC)

PAC-1 Monoclonal Antibody (PAC-1), FITC, Invitrogen™

WebCells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated ... WebConjugated Antibodies for IF ... FITC Plus . HRP . PE . Clear Confirms. Conjugate. 0. Alexa Fluor® 488 Alexa Fluor® 568 ... The most efficient cooling rate is 1°C per 1 minute, which can become achieved of putting vials into a commercial cryofreezing container or with wrapping them in ampere thick layer of cotton wool and placed in a -80°C ... rbl bank number of customersWebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 … rbl bank park street branch

"Web2. Slowly add FITC to protein with stirring. Cover with foil and stir 2 hours at room temperature. 3. Separate conjugate from free FITC on G-25 column. Collect fractions. 4. Pool fractions containing conjugate. 5. Determine F/P ratio of conjugate spectrophotometrically. 6. Stabilize with 1% bovine serum albumin and 0.1% sodium … " - Fitc-conjugated pac-1

Fitc-conjugated pac-1

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WebThe FITC-conjugated antibodies employed in a laboratory for the DFA test may be developed in house or commercially supplied. Some laboratories use a cocktail of monoclonal antibodies raised in mice against prominent antigens (e.g., nucleoprotein or glycoprotein), while others use a polyclonal antirabies antiserum raised in suitable … Webphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and ﬂuorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA).

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WebNov 19, 2014 · FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2 were prepared, and their integrin αvβ3/αvβ5 binding affinity was determined using the displacement assay against (125)I-echistatin bound to U87MG glioma cells. IC50 values of FITC-Galacto-RGD2, FITC-3P-RGD2, and FITC-RGD2 were calculated to be 28 ± 8, 32 ± 7, and 89 ± 17 nM, … WebThe PAC-1 antibody reacts with activation-induced conformational epitope of the integrin a IIb ß 3. PAC-1 binding to fibrinogen is pH and Ca 2+ dependent, and will not bind to platelets that have been fixed. We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher.

Web(340507) BD™ FITC Mouse Anti-Human PAC-1 - BD Biosciences - CiteAb. 156 citations – A FITC-conjugated mouse monoclonal antibody, raised against Proteasome assembly chaperone 1 (Human), supplied by BD Biosciences. ... Conjugate FITC Target Proteasome assembly chaperone 1 (Human) UniProt O95456 - PSMG1_HUMAN. Gene PSMG1 … WebProduct Specific Information. The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on …

WebIHC-plus™ ADCYAP1R1 / PAC1 Receptor Antibody LS-A109382 is a pathologist validated IHC antibody FITC Rabbit anti-Human ADCYAP1R1 / PAC1 Receptor. Validated for IHC. Tested on 20 paraffin-embedded human tissues. ... Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.) Purification. Websupplied, and the antibody subclass for each FITC-conjugated antibody. The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. Antibody Concentration Amount Subclass Anti-His(C-term)-FITC 1 mg/mL 50 μl (25 reactions)† IgG 2b Anti-myc-FITC 1 mg/mL 50 μl (25 reactions)† IgG 1

WebKLH conjugated synthetic peptide derived from human AMPD3: Concentration - 100 μg (in 100 μl): 1 mg/ml: Form/Appearance: Lyophilized or Liquid: Conjugation: FITC: MW: 89kDa: UniProt ID: O08739, Q01432, O09178: Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation. rbl bank other nameWebApr 4, 2024 · PAC-1 Antibody (4O21) is available as both the non-conjugated anti-PAC-1 antibody form, as well as multiple conjugated forms of anti-PAC-1 antibody, including agarose, HRP, PE, FITC and … rbl bank platinum first debit cardWeball FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1" solutions. 5. Prepare the required dilution of FITC in 0.1 M carbonate-bicarbonate buffer as directed in ... rbl bank officeWebFeb 1, 2024 · Binding of the FITC-conjugated PAC-1 as well as the PE-conjugated anti-P-selectin was recorded as MFI measured on the PC beads minus MFI of the NC beads. After 6 months and 12 months a new batch of beads was used (indicated by the dashed lines). Data are presented as mean ± SD (n = 4) and linear regression lines are shown. ... sims 4 city living townWebFITC Conjugated Anti-PAC-1 Monoclonal Antibody (Clone:PAC-1) Share: Figure 1: Surface staining of PAC-1 on PHA-activated (1 hour) human peripheral blood with anti-PAC-1 (PAC-1) FITC. rbl bank pod credit rbl bank press conferenceWebThe PAC-1 Antibody FITC has been validated for the following applications: Flow Cytometry. Flow cytometry of PAC-1 in PHA-activated human peripheral blood. Sample was incubated with PAC-1 monoclonal antibody (Product # MA5-28564) conjugated to FITC. rbl bank owner