Fluorescein labeled soybean agglutinin sba
WebAbstract The voltammetric detection of soybean agglutinin (SBA) was investigated on the basis of an interaction between the lectin and a sugar. Because galactose and lactose combined with SBA, the sugars were labeled by a Schiff base with an … WebCytofluorometric studies with fluorescein-labeled SBA (FITC-SBA) revealed that the majority of both mouse and human bone marrow cells bind the lectin. Experiments …
Fluorescein labeled soybean agglutinin sba
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WebSodium fluorescein, an orange-red crystalline hydrocarbon (C20 H 12 O 5 Na), has a low molecular weight (376.27 Da) and readily diffuses through most of the body fluids and … Webwith radioactively labeled tumor cells from different leukemia and ... tin soybean agglutinin (SBA) and further removal of residual T ... SBA Binding Assays. Fluorescein-bound SBA (1.5 mg/ml, 10 ,l) was incubated with leukemia cells (1 x 106/0. 1 ml) for 15 min at 0?C. The cells were then washed three times with Pi/
WebAlexa Fluor 594 wheat germ agglutinin (WGA) Hoechst 33342 Detailed experimental protocols ( Image-iT LIVE Plasma Membrane and Nuclear Labeling Kit) Each kit provides … WebSep 1, 1982 · Binding of fluorescein isothiocyanate-labeled SBA to mycelial cell walls could be abolished by adding N-acetyl galactosamine or galactose. Purified SBA at concentrations of 150 to 300...
WebSoybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Web4.1. Fluoresceins. Fluorescein is the most frequently used fluorescent label in FIA because of its high absorptivity and quantum yield. The quantum yield is 0.85 when the molecule …
WebFluorescent lectins have been used to localize and detect cell-surface or intracellular glycoconjugates, making them versatile primary detection reagents for microscopy or …
WebRCA 120 (Ricinus communis agglutinin; 2 mg/ml) PNA ( Arachis hypogaea (peanut) agglutinin; 2 mg/ml. These lectins are supplied essentially free … how do spell scheduleWebMar 1, 1988 · Fluorescein-conjugated lectins were used to probe the tumor cell surface. It was found that the percentage of cells in the population able to bind high levels of the lectin, soybean agglutinin (SBA), increased from 11 to almost 80% in the highly metastatic, enriched cell populations. how much should a 4\u00278 female weighWebAug 1, 1988 · Fluorescein isothiocyanate-peanut agglutinin labeling of fixed, permeabilized glands after addition of 300 mM sucrose. Oxyntic cell canalicular labeling is eliminated with sucrose (a sugar for which PNA is not specific), indicating low-affinity PNAbinding sites in these membranes. how do spells workWebThe amount of soybean agglutinin (SBA) detectable by radioimmunoassay in seeds of resistant cultivars to Phytophthora megasperma var. sojae was approximately twice that of susceptible cultivars. ... Binding of fluorescein isothiocyanate-labeled SBA to mycelial cell walls could be abolished by adding N-acetyl galactosamine or galactose. Purified ... how do spell tomorrow in spanishWebPreliminary structure analysis revealed that FOs has an average molecular weight of 11.81 kDa and consists of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose,... how do sperm and eggs differ quizletWebFluorescein labeled Soybean agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining … how much should a 4\u00274 girl weighWebFluorescein labeled Soybean Agglutinin (SBA) Product description Composed of four subunits of approximately equal size, soybean agglutinin is a family of closely related … how do spell slots work with multiclassing