Gateway® bp clonasetm ii enzyme mix

Author: jtnn

August undefined, 2024

WebThe attB-E(z) primers were designed to clone into the pENTR-221 vector with the help of Gateway™ BP Clonase™ II Enzyme mix (Thermo Fischer-11789100). The variant …

Gateway pDONR Vectors - Thermo Fisher Scientific

WebBP Clonase® II Enzyme Mix The BP Clonase® II enzyme mix (Box 2) contains the followin g rea ents. Store Box 2 at –20°C for up to 6 months. For long-term storage, store at –80°C. Item Composition Quantity BP Clonase® II Enzyme Mix Proprietary 40 μl Proteinase K solution 2 μg/μl in: 10 mM Tris-HCl, pH 7.5 20 mM CaCl 2 50% glycerol 40 μl WebSep 17, 2024 · Make sure you do not mix up the BP and LR clonase enzyme mixes. While the former should be used to generate the entry clone, the latter is always used to construct the expression clone. If your resultant expression clone is going to be more than 10 kb, it helps to increase the LR clonase reaction time to 2-3 hours instead of one hour; you can ... dj ruzo

Gateway cloning technology

WebJan 27, 2024 · Expression vector construction transformationAccording GatewayLR Clonase TMII Enzyme Mix (Invitrogen) manufacturer’s instructions, overex- pression RNAitarget sequences were transferred from entry vectors pK7GWIWG2D,respectively (Cheng et al. 2014). recombinantplasmids were used coliTrans5a competent cells, … WebClonase enzyme mix selection guide Figure 4. The Gateway reactions. The scheme shows the four types of plasmids and enzyme mixes involved in Gateway cloning reactions. Red arrows represent the fragment of interest. Adapted from Katzen F (2007) Expert Opin Drug Discov 2(4):571–589. Entry clone Kan R attL1 attL2 Destination vector ccdB Amp ... WebRemove the 5 x LR-Clonase II Enzyme Mix from -20°C and vortex 2 x 2 sec This is exorbitant stuff don't abandon it until rot in the ice-bucket! Add 1 µl about the 5 whatchamacallit LR-Clonase Enzyme DOUBLE Mingle to the Gateway reaction and mixed well ; Store the catalyst mix and storing immediately at -80°C !!! dj russo

Gateway BP Clonase II Enzyme Mix - Illinois Institute …

Thermo Fisher Scientific - US

WebStore Gateway® BP Clonase™ II enzyme mix at -20ºC (non-frost-free freezer) for up to 6 months. For long-term storage, store at -80ºC. Components Supplied 20 rxns 100 rxns … WebVitamin C Serum Test 2024 • Die 7 besten Vitamin C Serums im Vergleich - RTL Online. View. dj rutaWebBP Clonase II enzyme format is single mix of both enzyme and buffer to ensure enzyme stability and enable convenient 10μL reaction set up with fewer pipetting steps, which both maximize recombination efficiency and minimize pippetting. Original format of BP Clonase enzyme is stand alone enzyme blend and reaction buffer provided in separate tubes. dj rustic

"Web通过实时荧光定量PCR检测该基因在鹅掌楸花芽、盛花期的叶芽、叶片、花瓣、雄蕊、雌蕊中的相对表达量。利用Gateway技术构建该基因的植物过表达载体，使用农杆菌GV3101介导的花絮浸染法转化拟南芥，获得T2代转基因植株，直接观察转基因植株表型。 " - Gateway® bp clonasetm ii enzyme mix

Gateway® bp clonasetm ii enzyme mix

Gateway Invitrogen Cloning Cassette Thermo Fisher Bioz

WebContains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps. Brand: Invitrogen™ 11791020. 335.30 EUR valid until 2024-04-30. Use promo code "18437" to get your promotional price. Additional Details : Weight : 0.00454kg. WebMar 16, 2024 · * 干冰运输、大包装及大批量的产品需酌情添加运输费用 * 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司客服。产品描述相关资料下载相关产品浏览记录请 …

Did you know?

WebThaw on ice the Invitrogen BP Clonase II enzyme mix for about 2 minutes. Vortex the BP Clonase II enzyme mix briefly twice (2 seconds each time). To each sample (Step 1, above), add 2 µl of BP Clonase II enzyme mix to the reaction and mix well by vortexing briefly twice. Microcentrifuge briefly. Return BP Clonase II enzyme mix to –20°C or ... WebMay 7, 2024 · Construct the entry clone through a BP reaction. Set up the BP reaction mixture to include: 50 ng of the attB-flanked DNA fragment, 50 ng of pDONR221 (containing attP flankings), 0.5 μL of Gateway™ BP Clonase™ II Enzyme mix, q.s. to 2.5 μL with 1× TE buffer (pH 8.0). Incubate the reaction mixture at 25 °C for 1–2 h.

WebGateway ® BP Clonase II enzyme mix provided on page . 7. Determine how much . att. B DNA and donor vector to use in the reaction . For optimal efficiency, we recommend using the following amounts of . att. B PCR product (or linearized B expression clone) and donor vector in a 10 μL BP recombination WebCatalog number: 11789100. Gateway™ BP Clonase™ II enzyme mix catalyzes the in vitro recombination of PCR products or subcloning DNA segments from clones (containing att … Gateway™ BP Clonase™ II enzyme mix catalyzes the in vitro recombination of … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes

WebGateway™ BP Clonase™ II enzyme mix includes proteinase K solution (2 µg/µl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when … WebThe following reagents are supplied with LR Clonase™ II Plus enzyme mix. Store at –20°C for up to 6 months. For long-term storage, store at –80 C. Item Composition Amount LR Clonase™ II Plus Enzyme Mix Proprietary 40 l Proteinase K solution 2 g/ l in: 10 mM Tris-HCl, pH 7.5 20 mM CaCl 2 50% glycerol 40 l BP Clonase™ II Enzyme Mix

http://www.untergasser.de/lab/protocols/bp_gateway_reaction_ii_v1_0.htm

WebInvitrogen™ BP Clonase™ enzyme mix. Both LR Clonase and BP Clonase enzyme mixes are supplied in easy-to-use master mix formats, ensuring consistency and reliability from reaction to reaction. Select the Destination vector Once you have cloned your gene of interest or DNA fragment into a Gateway™ vector, you can shuttle it to as many dj rv rock azamgarhWebStore Gateway® BP Clonase™ II enzyme mix at -20ºC (non-frost-free freezer) for up to 6 months. For long-term storage, store at -80ºC. Components Supplied 20 rxns 100 rxns … dj rvs mp3WebThermo Fisher gateway bp clonase ii enzyme mix Gateway Bp Clonase Ii Enzyme Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more dj rv salesWebFind gateway® bp clonase® ii enzyme mix - 11789020 and related products for scientific research at MilliporeSigma. US EN. Applications Products Services Support. Advanced … dj ruudWeb8. Remove the 5 x BP-Clonase TM II enzyme mix from -20°C. It is most efficiently mixed by pipetting up and down, do not vortex. 9. Pipett 1 µl of this BP-Clonase TM II enzyme mix to the Gateway ® reaction. This is expensive stuff don’t leave it to rot in the ice-bucket! 10. Store the enzyme mix and buffer immediately at -20°C !!! dj rv 7WebThermo Fisher Scientific - US dj rvmbaWebContent And Storage. Gateway™ BP Clonase™ II enzyme mix includes proteinase K solution (2 µg/µl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored. Shipping Condition. Dry Ice. dj rv storage