Web01. maj 2024. · The passage number of the LifeAct-tdTomato knocked-in E14tg2a cells used in this study was less than 40. 2.5. Live cell imaging and multiphoton laser ablation. To capture the dynamics of Life-Act-tdTomato knocked-in cells on the mesh substrates, we performed timelapse microscopy at an interval of 2 h for 60 h using LCV-MPE … WebU2OS cells expressing Lifeact-tdTomato and stained with MitoTracker Green. Top row: single-camera configuration. Bottom row: dual-camera configuration. Crosstalk is minimized. In addition, twice as many images can be acquired, resulting in a …
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WebBrand: AMICUS. Model: 20160768. LACTOACT TABLETS. Product description: LactoAct (lactose 3300 FCC) breaks down lactose entered food, and thus allows the consumption … Web20. apr 2024. · Lifeact, a 17 amino-acid peptide, when fused to tdTomato, stains F-actin structures without interfering actin dynamics in vitro or in vivo . Upon AMPK activation, increased F-actin remodeling was observed in WT cells as shown by increased Lifeact-tdTomato fluorescence under TIRFM ( Figures 5A, B and Supplementary Figure 5B and … cscl east china sea 055e
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WebPlasmid pLenti-Lifeact-tdTomato from Dr. Weiping Han's lab contains the insert Lifeact and is published in Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951. This … WebA membrane-localized tdTomato gene (Myr-tdTomato) was linked to a nuclear-localized EGFP gene (H2B-EGFP) with the 2A sequence. The tdTomato is a red fluorescent protein and contains a tandem repeat of two copies of dTomato (Shaner et al. 2004). The TAG expression and 2A function have been assessed both in developmental stages and in … Web20. apr 2024. · Lifeact, a 17 amino-acid peptide, when fused to tdTomato, stains F-actin structures without interfering actin dynamics in vitro or in vivo . Upon AMPK activation, increased F-actin remodeling was observed in WT cells as shown by increased Lifeact-tdTomato fluorescence under TIRFM ( Figures 5A, B and Supplementary Figure 5B and … dyson and vex gif