Phospho-sting antibody

WebThe Phospho-STING (Ser366) assay measures STING when phosphorylated at Ser366. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-STING (Ser366) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is … WebPhospho-STING (Ser366) Polyclonal Antibody Product Details Size 100 µL Species Reactivity Human, Mouse, Rat Host / Isotype Rabbit / IgG Class Polyclonal Type Antibody Conjugate Unconjugated Immunogen A synthesized peptide derived from human STING around the phosphorylation site of Ser366. Form Liquid Concentration 1mg/mL

Phosphorylation of innate immune adaptor proteins …

WebThe interaction between STING and NCOA4 exacerbates lethal sepsis by orchestrating ferroptosis and inflammatory responses in macrophages. In Cell Death & Disease on 28 July 2024 by Wu, J., Liu, Q., et al.. WebWestern blot analysis of extracts of THP-1 cells using Phospho-STING (Ser365) Polyclonal Antibody (Product # PA5-117271). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in … fish monster movie https://negrotto.com

Phospho-STING (Ser366) Polyclonal Antibody - Thermo …

WebWestern blot - Phospho-STING-S365 Rabbit pAb (AP1199) Western blot analysis of extracts of THP-1 cells, using Phospho-STING-S365 antibody (AP1199) at 1:1000 dilution.THP-1 … WebDec 6, 2024 · c Immunoblots of STING/IRF3 axis in the lung of WT and STING −/− mice, including phospho-STING, STING, phospho-TBK1, TBK1, phospho-IRF3, and IRF3, with β-actin as a reference. cancun all inclusive resorts packages cost

Phospho-Specific Antibodies for Flow Cytometry Thermo Fisher ...

Category:Phospho-STING-Ser366 antibody Anti-Phospho-STING-Ser366

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Phospho-sting antibody

Recombinant Anti-STING antibody [SP338] (ab227704)

WebFull length recombinant STING (TMEM173) protein produced in 293T cells. This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Affinity purified. Upon receipt, store undiluted between 2°C and 8°C. Each lot of this antibody is quality control tested by Western blotting. WebCell Signaling Technology's Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor ® 647 Conjugate) is a Rabbit monoclonal antibody. The Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor ® 647 Conjugate) Antibody was generated using stimulator of interferon response cGAMP interactor 1, and STING1 as the antigen. It reacts with …

Phospho-sting antibody

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WebHowever, detection of phosphorylated proteins in western blots can be challenging. At Bio-Rad, we have developed 10 tips to ensure robust data generation and cleaner blots. Topics include: Inhibition of protein degradation and conservation of post-translational modifications during sample preparation. Selection of phospho-specific antibodies. WebPhospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of STING protein only when phosphorylated at Ser366. Source/Purification: Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal condi-

WebAnti-STING Antibody Products. Listed below are anti-STING antibodies from multiple suppliers. STING is a reported alias name for the human gene TMEM173, or 'transmembrane protein 173'. The 379-amino acid protein is a member of the TMEM173 family. The cellular localization is predicted to be cytoplasmic, mitochondrial and membrane-associated. WebMar 25, 2024 · Storage : -20°C. Description : STING is a 379 amino acid containing signaling protein that acts as an important component of innate immune signaling. This signaling protein is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state ...

WebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization , Foxp3/Transcription Factor Buffer, and IC … WebMyBioSource.com's Phospho-STING (Ser366) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Immunohistochemistry, and Western Blot. Description: Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes …

WebAll lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution Lane 1 : Human THP-1 Whole Cell Extract Lane 2 : Mouse Total Lung Extract Lysates/proteins at …

WebWestern Blot analysis of Phospho-TBK1 (Ser172) performed on various cell lines using a Phospho-TBK1 (Ser172) monoclonal antibody ( Product # MA5-35869) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was … fish moochimWebMar 26, 2024 · Phospho antibodies can be used in either research or clinical applications. In clinical settings, the detection of a particular phosphorylated protein by a phospho … fish monthly clubWebInvitrogen Anti-Phospho-STING (Ser366) Polyclonal, Catalog # PA5-105674. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and … fish moodboardWebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. fish monthWebProduct name Anti-STING antibody [SP338] See all STING primary antibodies Description Rabbit monoclonal [SP338] to STING Host species Rabbit Tested applications Suitable … cancun all inclusive with private poolWebThe Human Monocyte STING Activation Flow Cytometry Panel includes antibodies targeting phospho-STING (Ser366) and phenotyping markers to enable observation of STING activation in human monocyte populations among peripheral blood mononuclear cells (PBMCs). CD45 is a pan leukocyte marker. Monocytes and dendritic cells (DCs) are … fish montrealWebPhospho-STING (Ser366) Antibody recognizes endogenous levels of STING protein only when phosphorylated at Ser366. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to … fish mood board