Photocleavable spacer
WebAug 1, 1995 · While the strong biotin-avidin interaction has been widely used for the detection of biomolecules, its irreversibility complicates their isolation. We report the synthesis of a photocleavable biotin derivative (PCB) which eliminates many limitations of existing methods. This reagent contains a biotin moiety linked through a spacer arm to a ... WebPhotocleavable Modifications. Explore our photocleavable analogues of the Amino- and Spacer-Modifiers and the Biotin label, as well as our PC linker molecule. The general design of the PC monomers is based on an α-substituted 2-nitrobenzyl group. The photo-reactive group is derivatised as a cyanoethyl phosphoramidite that can be incorporated ...
Photocleavable spacer
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WebConveniently introduce spacer modifications into your oligonucleotide during synthesis with our high-quality spacer phosphoramidites and solid supports. Adding a spacer adds … WebPC Spacer (photocleavable) is a non-nucleosidic molety that can be used as an intermediary to attach any available phosphoramidite modification at either end of an oligonucleotide …
WebMar 19, 2003 · New cationic lipids having an o-nitrobenzyl moiety as a photocleavable spacer between its hydrophilic and hydrophobic region were synthesized. To improve the efficiency of transfection with lipoplexes, … WebThe Vertiflex Procedure is redefining the treatment of LSS for patients. It provides patients with a clinically proven, minimally invasive solution that is designed to deliver long-term relief from the leg and back pain associated …
http://genelink.com/newsite/products/mod_detail.asp?modid=18 WebBio-Synthesis offers photocleavable PC Biotin Oligo Modification at various scales. This light activatable PC biotin modified oligonucleotide contained a photocleavable spacer between the biotin group and the DNA bases. The biotin group is separated from the 5' end nucleotide base by the photo-cleavable group and a long chain spacer arm.
WebAug 1, 1995 · This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any substrate. In experiments using [leucine]-enkephalin as a model substrate, we show that PCB retains its high affinity toward avidin/streptavidin and allows rapid (< 5 min) and …
WebAug 1, 1995 · This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any … lindsay horvathWebPhotocleavable spacer modified oligonucleotides could be used to tether an antisense oligo to a dextran, which is too large to pass through the nuclear pores. Photocleavage would … lindsay hopkins guilford cthttp://genelink.com/newsite/products/mod_detail.asp?modid=134 lindsay hopkins defiance ohioWebFeb 5, 2010 · DNAzymes incorporating photocleavable spacers were switched “on” or “off” with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable ... lindsay horvath staffWebAug 2, 2024 · Figure 3: A photocleavable biotin spacer useful for phosphoramidite oligonucleotide synthesis. This spacer allows the capture of modified oligonucleotides using the streptavidin system and the rapid, quantitative removal of the biotin spacer using near UV light at 300 to 350 nm. The released oligonucleotides are now suitable for other ... hot logic microwaveWebPC Spacer is a photocleavable C3 spacer modifier. Our ability. We provide a flexible ordering model to quickly meet your order requirements, whether it is a large number of standard products or customized products. With a GLP laboratory and unique technology development, we can help you meet the ever-changing market needs. ... lindsay houffWebPhotocleavable PC linker contained a non-nucleosidic group that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 spacer arm. Upon … hot logic macrowave